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StudiesStudy Workflow

Study Workflow

Studies coordinate the path from sample collection to public submission. This page covers the study lifecycle and the key workflow steps.

Study Lifecycle

Created (Draft) → Samples assigned → Metadata completed → Marked ready for submission → Pipelines run (optional) → Submitted to ENA → Published (with accession IDs)

Draft Stage

A newly created study starts in draft state:

  • submitted: false
  • readyForSubmission: false
  • Samples can be added and removed
  • Metadata can be edited freely

Ready for Submission

When all metadata is complete, set readyForSubmission: true. This:

  • Records a readyAt timestamp
  • Signals to the facility that the study is ready for review
  • Does not prevent further editing

Submitted

After successful ENA submission:

  • submitted: true with submittedAt timestamp
  • studyAccessionId is set (e.g., PRJEB...)
  • Each sample receives its own accession numbers
  • The study becomes read-only for most fields

Test Submission

SeqDesk supports test submissions to the ENA development server:

  • testRegisteredAt records when a test submission was made
  • Test submissions expire after 24 hours on ENA’s side
  • Use test mode to validate everything before production submission

Running Pipelines from a Study

Studies are the launch point for bioinformatics pipelines:

  1. Open the study detail page
  2. Navigate to the Pipelines tab
  3. Select a pipeline (e.g., MAG)
  4. Choose which samples to include
  5. Configure pipeline parameters
  6. Launch the run

The pipeline system automatically generates a samplesheet from the study’s samples and their assigned reads. See Running a Pipeline for details.

Pipeline Results

After a pipeline completes, results are linked back to the study:

  • Assemblies — assembled contigs linked to individual samples
  • Bins — genome bins with completeness and contamination scores
  • Artifacts — QC reports, MultiQC summaries, and other outputs

Results appear in the study’s Pipelines tab and on individual sample pages.

Internal Notes

Studies support internal notes with edit tracking:

  • Any user with access can add or edit notes
  • The system tracks who edited the notes and when
  • Notes are visible on the study overview tab

Deleting a Study

Studies can be deleted if they have not been submitted to ENA:

  • Deleting a study unassigns all its samples (sets studyId to null)
  • The samples remain in their original orders
  • Pipeline runs associated with the study are preserved for reference
  • Submitted studies cannot be deleted