MIxS Checklist · ERC000028
Minimum information required for a prokaryotic pathogen sample
isolation_sourcedescribes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived
lat_longeographical coordinates of the location where the specimen was collected
collected_byname of persons or institute who collected the specimen
collection_dateThe date the sample was collected with the intention of sequencing, either as an instance (single point in time) or interval. In case no exact time is available, the date/time can be right truncated i.e. all of these are valid ISO8601 compliant times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10; 2008-01-23; 2008-01; 2008.
geographic_location_region_and_localityThe geographical origin of the sample as defined by the specific region name followed by the locality name.
identified_byname of the expert who identified the specimen taxonomically
environmental_sampleidentifies sequences derived by direct molecular isolation from a bulk environmental DNA sample (by PCR with or without subsequent cloning of the product, DGGE, or other anonymous methods) with no reliable identification of the source organism
mating_typemating type of the organism from which the sequence was obtained; mating type is used for prokaryotes, and for eukaryotes that undergo meiosis without sexually dimorphic gametes
geographic_location_country_andor_seaThe geographical origin of where the sample was collected from, with the intention of sequencing, as defined by the country or sea name. Country or sea names should be chosen from the INSDC country list (http://insdc.org/country.html).
host_health_stateHealth status of the host at the time of sample collection.
lab_hostscientific name of the laboratory host used to propagate the source organism from which the sample was obtained
host_scientific_nameScientific name of the natural (as opposed to laboratory) host to the organism from which sample was obtained.
bio_materialUnique identifier that references the biological material from which the sample was obtained and that ideally exists in a curated collection (e.g. stock centres, seed banks, DNA banks). The ID should have the following structure: name of the institution (institution code) followed by the collection code (if available) and the voucher id (institution_code:collection_code:voucher_id). Please note institution codes and collection codes are taken from a controlled vocabulary maintained by the INSDC: https://ftp.ncbi.nih.gov/pub/taxonomy/biocollections/
culture_collectionUnique identifier that references the culture (e.g. live microbial and viral cultures and cell lines) from which the sample has been obtained and that have been deposited in curated culture collections. The ID needs to provide an institution code and the culture id, with optional collection code, in the following structure: (-institution_code:(collection_code):voucher_id. Please note institution codes (and optional collection codes) are taken from a controlled vocabulary maintained by the INSDC: https://ftp.ncbi.nih.gov/pub/taxonomy/biocollections/
specimen_voucherUnique identifier that references the physical specimen that remains after the sequence has been obtained and that ideally exists in a curated collection. The ID should have the following structure: name of the institution (institution code) followed by the collection code (if available) and the voucher id (institution_code:collection_code:voucher_id). Please note institution codes and collection codes are taken from a controlled vocabulary maintained by the INSDC: https://ftp.ncbi.nih.gov/pub/taxonomy/biocollections/
isolateindividual isolate from which the sample was obtained
sub_speciesname of sub-species of organism from which sample was obtained
sub_strainname or identifier of a genetically or otherwise modified strain from which sample was obtained, derived from a parental strain (which should be annotated in the strain field; sub_strain from which sample was obtained
serovarserological variety of a species (usually a prokaryote) characterized by its antigenic properties
strainName of the strain from which the sample was obtained.