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Sequel IIe system

Established HiFi sequencer with direct on-instrument processing

PacBioSequel IIe1 GbE or 10 GbE copper
Manufacturer Page
2Flow Cells
3Kits
2Software
4Features

Features

  • Direct on-instrument HiFi processing
  • Up to eight SMRT Cells 8M in one run
  • Flexible sequencing times
  • Mature HiFi workflows

Compatible Flow Cells

SMRT Cell 8M Tray
101-389-001smrt-cell
Sequel II Sequencing Kit 2.0
101-820-200sequencing-plate

Compatible Kits

SMRTbell Prep Kit 3.0
102-182-700ligationDNA
HiFi Prep Kit 96
103-381-200ligationDNA
HiFi Plex Prep Kit 96
103-381-300ligationDNA

Compatible Software

SMRT Link
control

PacBio web-based end-to-end workflow manager for run setup, monitoring, analysis, and data management

SMRT Analysis
analysis

PacBio analysis module suite for secondary analysis and visualization of HiFi data

Sequel IIe Platform

PacBio

Sequel IIe is an established PacBio HiFi platform that supports accurate long-read sequencing with onboard processing.

Specifications

Read LengthHiFi long reads
Output~30 Gb per SMRT Cell
AccuracyHiFi >99%
SizeBenchtop / Production

Strengths

  • Mature installed base
  • On-instrument HiFi processing
  • Up to eight SMRT Cells per run

Considerations

  • Sales ended
  • Lower throughput than Revio
  • Large footprint

Best For

Existing HiFi labsLegacy workflowsSmaller production queuesMigration planning

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