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Revio system

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Revio system

Four-stage high-throughput HiFi long-read system

PacBioRevio1 GbE or 10 GbE copper
Manufacturer Page
2Flow Cells
4Kits
3Software
4Features

Features

  • Four independent sequencing stages
  • Onboard compute with Google DeepConsensus
  • Methylation calling in every run
  • SPRQ chemistry for simplified prep

Compatible Flow Cells

Revio SMRT Cell Tray
102-202-200smrt-cell
Revio SPRQ Sequencing Plate
103-504-900sequencing-plate

Compatible Kits

Revio SPRQ HiFi Prep Kit 96
103-522-600ligationDNA
HiFi Prep Kit 96
103-381-200ligationDNA
HiFi Plex Prep Kit 96
103-381-300ligationDNA
SMRTbell Prep Kit 3.0
102-182-700ligationDNA

Compatible Software

SMRT Link
control

PacBio web-based end-to-end workflow manager for run setup, monitoring, analysis, and data management

SMRT Link Cloud
control

PacBio-hosted cloud version of SMRT Link for run setup and monitoring without local server

SMRT Analysis
analysis

PacBio analysis module suite for secondary analysis and visualization of HiFi data

Revio Platform

PacBio

Revio is PacBio's high-throughput HiFi long-read system for population-scale genome, transcriptome, and epigenome work.

Specifications

Read LengthHiFi long reads
Output35-100 Gb per SMRT Cell
Accuracy99.95% (Q33)
SizeProduction

Strengths

  • HiFi long-read accuracy
  • Four independent stages
  • Onboard compute

Considerations

  • High capital and infrastructure
  • Vendor-specific consumables
  • Complex run planning

Best For

High-confidence assembliesSV/SNV phasingPopulation-scale HiFiMethylation-aware studies

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