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PromethION 48

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PromethION 48

Production-scale PromethION with 48 flow-cell positions

Oxford NanoporePromethIONIntegrated compute and DAU; Ethernet
Manufacturer Page
2Flow Cells
14Kits
2Software
5Features

Features

  • 48 independently addressable flow-cell positions
  • 60 TB SSD and 512 GB RAM
  • 4 NVIDIA Ampere GPUs plus display GPU
  • Highest ONT throughput
  • DNA and RNA sequencing

Compatible Flow Cells

PromethION Flow Cell R10.4.1
FLO-PRO114Mpromethion
PromethION Flow Cell RNA
FLO-PRO004RApromethion-rna

Compatible Kits

Ligation Sequencing Kit V14
SQK-LSK114ligationDNA
Ligation Sequencing Kit XL V14
SQK-LSK114-XLligationDNA
Ultra-Long DNA Sequencing Kit V14
SQK-ULK114ligationDNA
Multiplex Ligation Sequencing Kit XL V14
SQK-MLK114.96-XLligationDNA
Rapid Sequencing Kit V14
SQK-RAD114rapidDNA
Rapid Barcoding Kit 24 V14
SQK-RBK114.24barcodingDNA
Rapid Barcoding Kit 96 V14
SQK-RBK114.96barcodingDNA
Rapid PCR Barcoding Kit 24 V14
SQK-RPB114.24pcrDNA
Native Barcoding Kit 24 V14
SQK-NBD114.24barcodingDNA
Native Barcoding Kit 96 V14
SQK-NBD114.96barcodingDNA
16S Barcoding Kit 24 V14
SQK-16S114.24ampliconDNA
cDNA-PCR Sequencing Kit V14
SQK-PCS114cdnaRNA
cDNA-PCR Barcoding Kit V14
SQK-PCB114.24cdnaRNA
Direct RNA Sequencing Kit
SQK-RNA004direct-rnaRNA

Compatible Software

MinKNOW
control

Device control, data acquisition, and basecalling software

Dorado
basecalling

High-performance basecalling server

PromethION Platform

Oxford Nanopore

PromethION extends nanopore sequencing from small benchtop deployments to production-scale operation, spanning P2 Solo through P24 and P48 models.

Specifications

Read LengthShort to ultra-long (>4 Mb)
OutputTerabases per run
Accuracy>99% (Q20+)
SizeBenchtop / Production

Strengths

  • Massively parallel flow-cell capacity
  • Higher per-flow-cell output
  • Production-scale throughput
  • Flexible scaling

Considerations

  • Higher capital cost
  • Larger space and power requirements
  • P2 Solo requires external compute

Best For

Population genomicsReference genomesLarge cohort sequencingCore facilities
$$$$1–3 days turnaround

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