Designed for quick turnaround and smaller experiments
Compatible Flow Cells
Flongle Flow Cell
FLO-FLG114flongle
Compatible Kits
Ligation Sequencing Kit V14
SQK-LSK114ligationDNA
Ligation Sequencing Kit XL V14
SQK-LSK114-XLligationDNA
Ultra-Long DNA Sequencing Kit V14
SQK-ULK114ligationDNA
Multiplex Ligation Sequencing Kit XL V14
SQK-MLK114.96-XLligationDNA
Rapid Sequencing Kit V14
SQK-RAD114rapidDNA
Rapid Barcoding Kit 24 V14
SQK-RBK114.24barcodingDNA
Rapid Barcoding Kit 96 V14
SQK-RBK114.96barcodingDNA
Rapid PCR Barcoding Kit 24 V14
SQK-RPB114.24pcrDNA
Native Barcoding Kit 24 V14
SQK-NBD114.24barcodingDNA
Native Barcoding Kit 96 V14
SQK-NBD114.96barcodingDNA
16S Barcoding Kit 24 V14
SQK-16S114.24ampliconDNA
cDNA-PCR Sequencing Kit V14
SQK-PCS114cdnaRNA
cDNA-PCR Barcoding Kit V14
SQK-PCB114.24cdnaRNA
Direct RNA Sequencing Kit
SQK-RNA004direct-rnaRNA
Compatible Software
MinKNOW
control
Device control, data acquisition, and basecalling software
Dorado
basecalling
High-performance basecalling server
Flongle Platform
Oxford Nanopore
Flongle is a low-cost small-scale ONT workflow that uses a disposable single-use flow cell inserted into the Flongle adapter on a compatible host device.
Specifications
Read LengthAny length
OutputUp to 2.8 Gb
Accuracy>99% (Q20+)
SizeAccessory
Strengths
Lowest-cost ONT entry per run
Fast setup
Good for QC and validation
Considerations
Low throughput
Requires adapter and host device
Single-use consumable
Best For
QC and verificationSmall amplicon jobsQuick sample checksTeaching and demos
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