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NextSeq 1000 System

Mid-throughput dry instrument with P1/P2 flow-cell support

IlluminaNextSeq 1000/20002 x Ethernet; 1 x USB 3.0
Manufacturer Page
2Flow Cells
3Kits
2Software
4Features

Features

  • Integrated DRAGEN secondary analysis
  • Supports P1 and P2 flow cells
  • Self-contained dry instrument design
  • Broad application support

Compatible Flow Cells

NextSeq 1000/2000 P1 Flow Cell
P1nextseq
NextSeq 1000/2000 P2 Flow Cell
P2nextseq

Compatible Kits

Illumina DNA Prep
20060060tagmentationDNA
Illumina RNA Prep with Enrichment
20040536tagmentationRNA
Nextera XT DNA Library Preparation Kit
FC-131-1024tagmentationDNA

Compatible Software

DRAGEN
analysis

Illumina secondary analysis platform for rapid genomic pipelines, including integrated on-instrument use

BaseSpace Sequence Hub
analysis

Cloud-based run management, storage, and analysis environment for Illumina sequencing

NextSeq 1000/2000 Platform

Illumina

NextSeq 1000 and NextSeq 2000 are mid-throughput Illumina platforms that combine flexible flow-cell options with integrated DRAGEN secondary analysis.

Specifications

Read LengthUp to 2 x 300 bp
Output10 - 540 Gb
Accuracy≥85-90% bases ≥Q30
SizeBenchtop

Strengths

  • Flexible P1-P4 flow-cell range
  • Integrated DRAGEN analysis
  • Broad application fit

Considerations

  • Higher capital cost
  • P3/P4 are NextSeq 2000 only
  • Less economical at very high scale

Best For

ExomesRNA-seqSingle-cellSpatial genomicsMedium-scale production
$$$1–2 days turnaround

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