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MiSeq Sequencing System

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MiSeq Sequencing System

Compact all-in-one SBS instrument for targeted and small runs

IlluminaMiSeqEthernet; front USB ports
Manufacturer Page
4Flow Cells
2Kits
3Software
4Features

Features

  • Single-instrument cluster generation, sequencing, and analysis
  • Up to 2 x 300 bp reads
  • Broad fit for amplicon and microbial work
  • Local Run Manager and BaseSpace integration

Compatible Flow Cells

MiSeq Reagent Nano Kit v2 Flow Cell
MS-103-1001miseq
MiSeq Reagent Micro Kit v2 Flow Cell
MS-103-1002miseq
MiSeq Reagent Kit v2 Flow Cell
MS-102-2002miseq
MiSeq Reagent Kit v3 Flow Cell
MS-102-3001miseq

Compatible Kits

Illumina DNA Prep
20060060tagmentationDNA
Nextera XT DNA Library Preparation Kit
FC-131-1024tagmentationDNA

Compatible Software

MiSeq Reporter
analysis

Pre-installed MiSeq software that performs secondary analysis after the sequencing run

Local Run Manager
control

Integrated Illumina software for run setup, monitoring, local analysis, and results viewing

BaseSpace Sequence Hub
analysis

Cloud-based run management, storage, and analysis environment for Illumina sequencing

MiSeq Platform

Illumina

MiSeq is Illumina's compact all-in-one SBS platform for targeted panels, amplicons, microbial genomes, and small projects.

Specifications

Read Length2 x 300 bp
Output300 Mb - 15 Gb
Accuracy>70-90% bases ≥Q30
SizeBenchtop

Strengths

  • Long SBS read-length option
  • Compact all-in-one workflow
  • Strong amplicon and microbial fit

Considerations

  • Lower throughput
  • Platform being made obsolete
  • Slower runtime

Best For

Amplicon sequencingMicrobial genomesPilot studiesEducation labs
$$1–2 days turnaround

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