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iSeq 100 Sequencing System

Compact cartridge-based SBS sequencer for very small runs

IlluminaiSeq 1001 x Ethernet; 2 x USB; Wi-Fi
Manufacturer Page
1Flow Cell
2Kits
2Software
4Features

Features

  • Single-use cartridge-based workflow
  • Up to 2 x 150 bp reads
  • Local Run Manager on instrument
  • Cloud apps via BaseSpace Sequence Hub

Compatible Flow Cells

iSeq 100 i1 Flow Cell
20031371iseq

Compatible Kits

Illumina DNA Prep
20060060tagmentationDNA
Nextera XT DNA Library Preparation Kit
FC-131-1024tagmentationDNA

Compatible Software

Local Run Manager
control

Integrated Illumina software for run setup, monitoring, local analysis, and results viewing

BaseSpace Sequence Hub
analysis

Cloud-based run management, storage, and analysis environment for Illumina sequencing

iSeq 100 Platform

Illumina

iSeq 100 is a compact low-output SBS instrument suited to small projects, QC, and verification work.

Specifications

Read Length2 x 150 bp
OutputUp to 1.2 Gb
Accuracy>80-85% bases ≥Q30
SizeDesktop

Strengths

  • Smallest Illumina footprint
  • Simple cartridge-based workflow
  • Good for proof-of-principle

Considerations

  • Very low throughput
  • Platform being made obsolete
  • Limited scalability

Best For

Library QCPilot studiesVery small amplicon jobsTraining labs

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